Enter one of 4096 possible 6mers into the search field or any nucleotide sequence of less than 7 nucleotides.
Use positions 2-7 of the guide/antisense strand of the RNA duplex as the 6mer.
Type in the miRNA search field. It will auto-complete to show matching mature miRNAs. You can also use the miRNA accession number.
Each miRNA is color-coded. The colors are explained in the Info section below.
With show miRNAs enabled, you can click on a mature miRNA in the results to be taken to the mirbase.org page corresponding to its accession number.
This site allows to determine the activity of any siRNA, shRNA, or miRNA to be toxic through its 6mer seed sequence in human (HeyA8 - ovarian, H460 - lung and H4 - brain) and mouse (M565 - liver, 3LL - lung and GL261 - brain) cancer cell lines.
The viability columns of the data table are colored as follows:
When show miRNAs is enabled, miRNAs are color-coded as follows:
Gao, Q.Q., Putzbach, W.E., Murmann, A.E., Chen, S., Sarshad, A.A., Peter, J.M., Bartom, E.T., Hafner, M. and Peter, M.E. (2018) 6mer seed toxicity in tumor suppressive microRNAs. Nature Comm, 9:4504.
Click for detailed changelogs.
This site was developed by Johannes Peter.
The source code is available on Github under the MIT license
You are welcome to file Bug Reports or make Feature Requests